Gene expression in mammalian cells largely depends on the epigenetic status of the chromatin, including DNA methylation, histone modifications, enhancer-promoter interactions, and noncoding RNA-mediated regulation. The coordinated actions of these multi-faceted regulations determine cell cycle regulation, cell fate, and the ultimate responses in development and disease. Therefore, studies of epigenetic modulations are critical for our understanding of gene regulation mechanisms at the cellular, tissue, and organ levels. Noncoding RNA and epigenetics research have seen explosive growth in recent years, but these fields are still young and poised for major advances in the near future. The aim of this symposium is to provide basic and clinical researchers with an overview of the principles of epigenetics, study methods, and recent findings in epigenetic and non-coding RNA-mediated regulation in cardiovascular disease. Studies using computational and high throughput sequencing tools for epigenetics analysis are also expected, with an emphasis on the cardiovascular system. This symposium will conclude with the perspectives of the application of biomedical engineering to advance epigenetics and the utilization of epigenetics to translate bioengineering research into clinical medicine.